SBFI AM ( Na+ Indicator)   鈉離子指示探針

【務必注意】：初次使用離子探針的用戶，強烈建議配合：Pluronic F-127, Cell Culture Tested 細胞培養級（MS4301-1G）一起使用，以提高探針的水溶性和胞內加載性。 

產品標簽

SBFI AM 鈉離子指示劑/探針，PBFI AM 鉀離子指示劑/探針，SQI-Et (Na) 鈉離子載體，Monensin 莫能霉素，CAS NO：129423-53-6

產品信息

【溫馨提示】：見我司懋康生物（MKBio）整理的鉀離子（K+）熒光探針專題。

【溫馨提示】：見我司懋康生物（MKBio）整理的鈉離子（Na+）熒光探針專題。

【溫馨提示】：見我司懋康生物（MKBio）整理的鈣離子（Ca2+）熒光探針專題。

產品描述

SBFI，英文全名Sodium-binding Benzofuran Isophthalate，一種Na+選擇性熒光指示劑，可用來預測純化線粒體Na+梯度，檢測胞內Na+水平，測定細胞Na+外流，以及與其他熒光指示劑聯合使用以分析Na+與Ca2+和Mg2+濃度、胞內pH和膜電位變化的相關性。雖然SBFI對Na+的選擇能力弱于Ca2+指示劑比如Fura-2，但在其他單價陽離子存在體系，SBFI足以檢測Na+的生理濃度。結合離子后的SBFI光譜反應可通過激發光比率測定來判定，其能與用相同光濾片和儀器檢測的探針Fura-2共同使用。

當體系內含生理濃度的K+/Na+（~135mM），SBFI對Na+的解離常數（Kd）為11.3mM；而不存在K+體系，對Na+的Kd為3.8mM。SBFI對Na+的選擇性比K+約強18倍。

本品為乙酰氧基甲基酯（Acetoxymethyl ester, AM ester）形式的SBFI，CAS NO：129423-53-6，具有細胞膜滲透性，只需簡單孵育即可進入細胞，常用加載濃度范圍5-10μM，加載時間40min-4h，根據具體的實驗要求和細胞類型來調整。

產品特性

1）化學名：4,4’-[1,4,10-trioxa-7,13-diazacyclopentadecane-7,13-diylbis(5-methoxy-6,2-benzofurandiyl)]bis-1,3- benzenedicarboxylic acid 1,1’,3,3’-tetrakis[(acetyloxy)methyl] ester

2）同義名：Sodium-binding Benzofuran Isophthalate Acetoxymethyl ester

3）CAS NO：129423-53-6

4）分子式：C56H58N2O23

5）分子量：1127.07

6）純度：＞90%（HPLC） 

7）Ex/Em：340,380/500 nm

8）外觀：淺黃至暗黃至暗橙固體或油狀

9）溶解性：溶于DMSO（10mM）

10）化學結構式：

保存與運輸方法

保存：-20℃避光干燥穩定凍存二年。

運輸：冰袋運輸。

注意事項

1）SBFI AM易受潮，粉末需要干燥保存；需用無水DMSO溶解，配制儲存液（如10mM），置于-20℃干燥避光，小量分裝避免反復凍融，至少3個月穩定。

2）由于SBFI AM水溶性較差，可在實驗前與等體積Pluronic F-127（25% w/v）混合，以提高探針加載效率。

3）為了您的安全和健康，請穿實驗服并戴一次性手套操作。

 — —Written/Edited by V. Shallan【版權歸MKBio懋康所有】

上海懋康生物科技有限公司是一家涉足于生命科學和生物技術領域研究的試劑、儀器和實驗室消耗品與實驗服務工作，主要從事細胞生物學、植物學、分子生物學、免疫學、生物化學、蛋白組學。生物制藥與診斷試劑研發生產等領域。 本公司秉承“以人為本，以誠為信、合同守信”的經營理念。堅持"品質保障"的原則為廣大客戶提供優質產品。

文獻引用： 

[1]

Tong W et al. Phthalocyanine functionalized poly(glycidyl methacrylate) nano-assemblies for photodynamic inactivation of bacteria. Biomater. Sci., 2019,7, 1905-1918

Sodium-binding benzofuran isophthalate acetoxymethyl ester (SBFI-AM) andPotassium-binding Benzofuran Isophthalate Acetoxymethyl ester (PBFI-AM) were purchased from MKbio. China.

[2]

Li R et al. Biofilm inhibition and mode of action of epigallocatechin gallate against Vibrio mimicus. Food Control, Volume 113, July 2020, 107148

Then PBFI probe (ShangHai MaoKang Bio technology Co., LTD., China) was added and incubated at 37 °C for 90 min.The cells were washed, collected and resuspended with PBS buffer. Aliquots (100 μL) of bacterial suspension were transferred to a Corning 96 well black plate, and 100 μL of various concentrations of EGCG were dispensed in the microtiter plate wells.

[3]

Liu Y, Zhen W, Wang Y, Song S, Zhang H. Na2S2O8 Nanoparticles Trigger Antitumor Immunotherapy through Reactive Oxygen Species Storm and Surge of Tumor Osmolarity. J Am Chem Soc. 2020 Dec 30;142(52):21751-21757. doi: 10.1021/jacs.0c09482. Epub 2020 Dec 18. PMID: 33337859.

4T1 cells were inoculated into glass bottom culture dishes for 24 h. Then, adding PNSO NPs medium solution (80 μg/mL) to continue co-culture for 4 h. The treated 4T1 cells were further incubated with 10

μM Na+ indicator SBFI AM (sodiumbinding benzofuran isophthalate acetoxymethyl ester, MKBio, 90%) in 0.04% Pluronic F-127 (MKBio) and the fluorescence signal was measured by CLSM.

[4] 

Liang Z, Yang Y, Yu G, et al. Engineering aluminum hydroxyphosphate nanoparticles with well-controlled surface property to enhance humoral immune responses as vaccine adjuvants. Biomaterials. 2021 Jun;275:120960. DOI: 10.1016/j.biomaterials.2021.120960.

[5] BMDMs were treated with AAHPs (250 μg/mL) in the presence of LPS at 500 ng/mL for 5 h.Then PBFI AM (Mkbio, Shanghai, China) was added to the cells at the concentration of 10 μM, and cells were incubated at 37 °C for 1h. Triton X-100 (0.2%) treated cells were used as controls. The fluorescence of PBFI AM was measured at the Ex/Em of 340/615 nm. The data were expressed as relative fluorescence intensity (RFI) defined as the fluorescence intensity of AAHPs-treated BMDMs normalized to the intensity of control cells.

[6] Jia Y, Yang B, Shi J, Fang D, Wang Z, Liu Y. Melatonin prevents conjugative transfer of plasmid-mediated antibiotic resistance genes by disrupting proton motive force. Pharmacol Res. 2022 Jan;175:105978. doi: 10.1016/j.phrs.2021.105978. Epub 2021 Nov 21. PMID: 34813930.

As for the detection of intracellular K + concentration, the PBFI-AM (K + indicator) fluorescence dye (Maokangbio, Shanghai, China) labeled cells (10 μM) in the presence of…..