FITC-Con A (FITC labeled Concanavalin A)

FITC標記刀豆蛋白A

點擊丨商城購買 積分領好禮

產品標簽

淋巴細胞轉化實驗（T Lymphocyte Transformation Test），非特異性有絲分裂原（mitogen），T細胞活化和增殖，Con A，Concanavalin A ，伴刀豆凝集素，植物血球凝集素PHA，CAS：11028-71-0

產品信息

植物凝集素系列相關產品：

1）  見我司懋康生物整理的植物血凝素PHA（PHA-L，PHA-M，PHA-E，PHA-P）產品專題和信息。

2）  見我司懋康生物整理的番茄凝集素Tomato-Lectin產品專題和信息。

3）  見我司懋康生物整理的加納籽凝集素I-同工凝集素B4（GSL I-B4，BSL I-B4）產品專題和信息。

4）  見我司懋康生物整理的刀豆蛋白凝集素Concanavalin A產品專題和信息。

5）  更多凝集素產品逐漸開發中。。。。。。

產品描述

刀豆蛋白A（Concanavalin A，Con A），來源于Canavalia ensiformis洋刀豆（刀豆），是一種凝集素蛋白（Mw 104kDa）。pH≥7.0條件下，Con A以同質四聚體結構形式存在，由四個分子量為26kDa的亞基構成。酸性條件（pH 4.5-5.5）下，Con A解離為活化的二聚體結構（52kDa）。每一個單體，不管pH值或分子結構如何，都包含2個金屬離子結合位點。金屬離子（Ca2+ 和 Mn2+ ）必須結合到這兩個位點上Con A才能發揮活性。乙?；?、琥珀?；蚱渌苌镆材墚a生穩定的二聚體結構形式。

刀豆蛋白A（Concanavalin A，Con A）無血型特異性，但選擇性結合發現于各種糖蛋白、糖脂和糖類分子上的α-甘露糖基（α-mannosyl residues）和α-葡糖基殘基（α-glucosyl residues），需要Ca2+ 和 Mn2+ 離子存在才能顯示活性。Con A適用于碳水化合物研究，糖蛋白純化，酶標記（enzyme tagging），細胞膜研究，細胞凝集，和細胞分型研究。

本品是FITC標記的刀豆蛋白（FITC labeled Concanavalin A, FITC-ConA），Ex/Em=495/515nm，親和純化所得，基本不含未標記的熒光素。建議工作濃度范圍是5-20μg/ml。

產品特性

1）   英文同義名：Concanavalin A from Canavalia ensiformis (Jack bean), FITC Conjugate; FITC-ConA;

FITC labeled Concanavalin A (ConA); ConA lectin (FITC);

2）   中文同義名：刀豆蛋白 A 來源于洋刀豆 （刀豆），FITC標記；FITC標記的刀豆凝集素A；FITC標記的刀豆球蛋白A；FITC標記的伴刀豆球蛋白A；

3）   糖類特異性：α-甘露糖；α-葡萄糖；

4）   F/P（摩爾）：2.0-6.0

5）   外觀：溶液（溶于10 mM HEPES, 0.15 M NaCl, pH 7.5, containing 0.1 mM Ca2+, 0.08% sodium azide and 0.01mM Mn2+）

6）   蛋白濃度：5mg/ml

7）   Ex/Em：495/515nm

8）   應用：IF、糖生物學

保存與運輸方法

保存：2-8℃避光保存，至少1年有效。

運輸：冰袋運輸。

注意事項

1）   本品置于2-8℃長期保存可能會產生沉淀，使用前請37℃溫育數分鐘，之后離心吸取上清使用。此操作基本不會對產生性能造成負影響。

2）   為了您的安全和健康，請穿實驗服并戴一次性手套操作。 

常見問題

1）   FITC標記刀豆蛋白（FITC-ConA）（# MP6321-5MG）的建議工作濃度是多少？ 如何配制染色工作液？

回復：本品用于免疫熒光染色（IF）的建議工作濃度是5-20μg/ml，用戶可根據自身的實際需求或參考文獻來進行工作濃度的優化?？捎蒙砭彌_液如PBS，HEPES等稀釋到工作濃度。

2）   FITC標記刀豆蛋白（FITC-ConA）（# MP6321-5MG）進行免疫熒光染色（IF）的建議孵育條件如何？

回復：一般孵育條件是室溫（RT）孵育30min，也可于4℃孵育30-1h。具體的孵育條件請根據自身的實際需求或參考文獻來進行優化。

相關文獻a）The Candida albicans ENO1 gene encodes a transglutaminase involved in growth, cell division, morphogenesis, and osmotic protection. PMID: 29386353. 【Cells were washed twice with 1× PBS, permeabilized with 70% ethanol, incubated with 2 μg/ml FITC-ConA in 1× PBS for 30 min at room temperature, washed twice with 1× PBS, and analyzed in the flow cytometer. The maximum excitation wavelength was 495 nm, and the maximum emission wavelength was 515 nm.】

相關文獻b）Staining of the inner acrosomal membrane of human spermatozoa with concanavalin A lectin as an indicator of potential egg penetration ability. PMID: 1718787. 【Spermatozoa were pelleted by microcentrifugation, and excess fixative was removed. The pellet was resuspended in a small volume of fixative remaining and 20μl aliquots of fixed sperm suspension were allowed to air dry on poly-L-lysine-treated slides. The slides were then rinsed in 0.2 M glycine followed by a second wash in 10 mM phosphate buffered saline (PBS). Spermatozoa were labeled with 25μl of 100μg FITC-ConA/mL in PBS for 25 minutes at RT. Slides were rinsed with excess PBS and mounted in medium16 before immediate examination at a magnification of 400X under an Olympus BHT microscope.】

3）   FITC標記刀豆蛋白（FITC-ConA）（# MP6321-5MG）如何進行視網膜血管染色？

回復：很多臨床前實驗表明在各種FITC標記的凝集素中刀豆蛋白A對視網膜血管（retinal vasculature）能表現出最均勻的染色結果。很多文獻用FITC-ConA來標記血管壁上粘附的白細胞和血管內皮細胞。使用方法可參考以下文獻：

文獻a）Simvastatin Inhibits Leukocyte Accumulation and Vascular Permeability in the Retinas of Rats with Streptozotocin-Induced Diabetes. PMID: 15111316.【Six different rats were used in each group. After the induction of deep anesthesia, the chest cavity was carefully opened and a 20-gauge perfusion cannula was introduced into the aorta. Drainage was achieved by opening the right atrium. The animals were then perfused with 100 ml of phosphate-buffered saline (PBS) to wash out blood cells in the vessels, such as erythrocytes and nonadherent leukocytes. After PBS perfusion, the animals were perfused with 25 ml of fluorescein isothiocyanate (FITC)-labeled concanavalin A lectin (ConA; 40 μg/ml in PBS, pH 7.4). ConA was used to label leukocytes adherent to the vessel walls. Residual unbound ConA was removed with PBS perfusion. The retina was carefully removed and fixed with 1% paraformaldehyde, and flat mounts were prepared using a fluorescence anti-fading medium. The retinas were then observed using fluorescence microscopy】

文獻b）VEGF164 Is Proinflammatory in the Diabetic Retina. PMID: 12714656 【Perfusion with FITC-ConA or Rhodamine-Con A (40 μg/mL in PBS [pH 7.4], 5 mg/kg BW) was then performed to label adherent leukocytes and vascular endothelial cells, followed by removal of residual unbound lectin with PBS perfusion.】

 — —Written/Edited by V. Shallan【版權歸MKBio懋康所有】

上海懋康生物科技有限公司是一家涉足于生命科學和生物技術領域研究的試劑、儀器和實驗室消耗品與實驗服務工作，主要從事細胞生物學、植物學、分子生物學、免疫學、生物化學、蛋白組學。生物制藥與診斷試劑研發生產等領域。 本公司秉承“以人為本，以誠為信、合同守信”的經營理念。堅持"品質保障"的原則為廣大客戶提供優質產品。

引用文獻：

[1]Zhu T, Xu L, Yang L, Zhao P, Zhu X, Wu Z, Song Z. Microbiologically influenced corrosion inhibition of carbon steel by a novel bacterium, Photobacterium sp., in simulated marine environment. Environ Res. 2024 Nov 5;264(Pt 1):120298. doi: 10.1016/j.envres.2024.120298. Epub ahead of print. PMID: 39510236.

The proteins, polysaccharides and bacteria (nucleic acids) on the coupon surface w

ere stained by fluorescein isothiocyanate (FITC) (20 μg/mL), Rhodamine labeled concanavalin A (25 μg/mL) (Maokangbio, Shanghai, China) and DAPI (Beyotime Biotechnology, Shanghai, China) for 10, 30, and 10 min in a dark room, respectively.