Biotinylated Lycopersicon Esculentum (Tomato) Lectin (LEL, TL)

生物素化番茄凝集素

產品標簽

番茄凝集素（Tomato lectin）；FITC-LEL；Intravascular perfusion 血管內灌注；Lectin perfusion 凝集素灌注；Microvasculature 微血管；Endothelial cells 內皮細胞；

產品信息【原裝正品，現貨供應，大量引用文獻支持】

【溫馨提示】：見我司（懋康生物）整理的Vector Labs血管功能研究-番茄凝集素產品專題。

產品描述

番茄凝集素（Tomato lectin，Lycopersicon Esculentum Lectin（LEL）），是一種非常穩定的單亞基糖蛋白，約含有等量的蛋白質和碳水化合物。據報道其糖類部分由85%阿拉伯糖和15%半乳糖組成，在溶液中可能形成多聚體聚合物。番茄凝集素雖然和土豆凝集素、曼陀羅凝集素（DSL）和麥胚凝集素（WGA）有部分相同的特異性，但在許多多面呈現出獨特之處。番茄凝集素LEL能夠很好地與血型糖蛋白（glycophorin）和Tamm-Horsfall糖蛋白結合，能夠有效結合和標記嚙齒類動物的血管內皮（vascular endothelium）。

本品為生物素化的番茄凝集素（來源于Lycopersicon esculentum），是一種理想的中間體檢測糖偶聯物，通過生物素-親和素/鏈霉親和素系統。首先加入生物素化凝集素，隨后使用VECTASTAIN® ABC，Avidin D conjugate或streptavidin derivative。

產品特征

使用方法（Histochemistry）【更多請點擊此處下載Lectin Immunohistochemistry, Elisa, Western Blots Guidelines】

1a. Staining procedure for paraffin sections:  Deparaffinize and hydrate tissue sections through xylenes or other clearing agents and graded alcohol series and rinse for 5 minutes in tap water.  If required, retrieve antigens using the Antigen Unmasking Solution (H-3300 or H-3301).

1b. Staining procedure for frozen sections:  Air dry sections.  Immediately before staining, fix sections with acetone.  Transfer slices to buffer.  If endogenous enzyme activities are present, inactivate using appropriate methods.

2. Perform Streptavidin/Biotin blocking if required following kit instructions (SP-2002).  Avidin in the Avidin/Biotin Blocking Kit (SP-2001) is a glycoprotein containing terminal mannose residues.  Use of the Avidin/Biotin Blocking Kit is not recommended especially when using mannose-specific lectins.

3. Block non-specific binding by incubating section with Carbo-Free? Blocking Solution (SP-5040) for  30 minutes at room temperature.  Blot excess blocking solution from the sections.

4. Apply biotinylated lectin at approximately 2-20 μg/ml in PBS (10 mM sodium phosphate, 150 mM NaCl, pH 7.4) to the sections and incubate for 30 minutes at room temperature.  Wash with TPBS  (PBS + 0.05% Tween? 20).

5. Prepare VECTASTAIN® ® ABC (peroxidase, PK-6100) or VECTASTAIN® ABC-AP (alkaline phosphatase, AK-5000) reagents according to the kit instructions.  Apply to the sections and incubate for 30 minutes at room temperature.  Wash with TPBS.

6. Apply an appropriate precipitating substrate for the enzyme system used in step 4.  For peroxidase, ImmPACT? DAB (SK-4105) is recommended; for alkaline phosphatase, ImmPACT? Vector® Red  (SK-5105).  Rinse in tap water.

7. Counterstain (optional), clear and mount.  For galactose or GalNAc-specific lectins we recommend that sections be mounted in VECTASHIELD® HardFSet? Mounting Medium (H-1400) or VECTASHIELD® HardFSet? Mounting Medium with DAPI (H-1500).

相關產品：

引用文獻【Vector Labs，Tomato Lectin】

Huang J et al. Regression of established tumors and metastases by potent vascular endothelial growth factor blockade. Proc Natl Acad Sci U S A. 2003 Jun 24;100(13):7785-90. PMID: 12805568【Vascular lectin perfusion, 100 μg in 100 μl of saline, fluorescein- labeled Lycopersicon esculentum lectin, Vector Lab】

Inai T et al. Inhibition of vascular endothelial growth factor (VEGF) signaling in cancer causes loss of endothelial fenestrations, regression of tumor vessels, and appearance of basement membrane ghosts. Am J Pathol. 2004 Jul;165(1):35-52. PMID: 15215160【Vascular lectin perfusion, 100 μg in 100 μl of 0.9% NaCl, fluorescein- labeled Lycopersicon esculentum lectin, Vector Lab】

Thurston, G., et al., Cationic liposomes target angiogenic endothelial cells in tumors and chronic inflammation in mice. J. Clin Invest, 1998. 101 (7): p. 1401-1413. PMID: 9525983

Hashizume, H., et al., Openings between defective endothelial cells explain tumor vessel leakiness. Amer J Pathol, 2000. 156(4): p. 1363-1380. PMID: 10751361.【Vascular perfusion, biotin- or fluorescein isothiocyanate (FITC)-labeled Lycopersicon esculentum lectin, 100 μg in 100 μl 0.9% NaCl, Vector Laboratories】   

Debbage, P.L., et al., Intravital lectin perfusion analysis of vascular permeability in human micro- and macro- blood vessels. Histochemistry Cell Biol, 2001. 116(4): p. 349-359.   

Lee, J.C., et al., Interleukin-12 inhibits angiogenesis and growth of transplanted but not in situ mouse mammary tumor virus-induced mammary carcinomas. Cancer Res, 2002. 62(3): p. 747-755.   

Akerman, M.E., et al., Nanocrystal targeting in vivo. Proc Nat Acad Sci Usa, 2002. 99(20): p. 12617-12621.   

Gee, M.S., et al., Tumor vessel development and maturation impose limits on the effectiveness of anti-vascular therapy. Amer J Pathol, 2003. 162(1): p. 183-193.   

Jilani, S.M., et al., Selective binding of lectins to embryonic chicken vasculature. J Histochem Cytochem, 2003. 51(5): p. 597-604.   

Krasnici, S., et al., Effect of the surface charge of liposomes on their uptake by angiogenic tumor vessels. Int J Cancer, 2003. 105(4): p. 561-567.

 — —Written/Edited by V. Shallan【版權歸MKBio懋康所有】

上海懋康生物科技有限公司是一家涉足于生命科學和生物技術領域研究的試劑、儀器和實驗室消耗品與實驗服務工作，主要從事細胞生物學、植物學、分子生物學、免疫學、生物化學、蛋白組學。生物制藥與診斷試劑研發生產等領域。 本公司秉承“以人為本，以誠為信、合同守信”的經營理念。堅持"品質保障"的原則為廣大客戶提供優質產品。