Fluorol Yellow 088  熒光黃088（木栓質染色）

點擊丨商城購買 積分領好禮

產品標簽

Fluorol Yellow 088 熒光黃088；Solvent Green 4 溶綠4；Suberin lamellae 木栓質層；Ruthenium Red 釕紅；Calcofluor White Stain 卡爾科弗盧爾熒光增白劑；CAS NO：81-37-8；

產品信息

產品描述

熒光黃088（Fluorol Yellow 088）是一種多環芳烴有機染料，一種親脂性熒光素，用于植物組織木栓質層的細胞染色。

產品特性

保存與運輸方法

保存：室溫避光干燥保存，2年有效。

運輸：室溫運輸。

應用實例

文獻1:Lux A, Morita S, Abe J, Ito K, 2005.An improved method for clearing and staining free-hand sections and whole-mount samples. Ann Bot (Lond) 96:989–996

A）Clearing solution：Lactic acid was saturated with chloral hydrate.

B）(a) A 0.1 % (w/v) solution of berberine hemisulphate (小檗堿) in lactic acid was dissolved at room temperature. (b) A 0.01 % (w/v) solution of fluorol yellow 088 (熒光黃088, MX4473) in lactic acid was prepared by heating at 70°C for 1h. Due to the low stability of the solution, this was prepared fresh before each use.

C) Clearing and staining procedure

The sections floating in drops of clearing solution on microscope slides were heated over a water bath in covered Petri dishes. Treatment for 1h, using a high temperature (70°C), even with large or dense sections, was usually sufficient. The clearing solution was then absorbed by pipette or tissue paper and the sections were thoroughly washed with distilled water added several times to the sections and absorbed by tissue paper. Sections were post-stained and subsequently washed in the same way. Framing by a liquid blocker (PAP pen) proved useful to prevent the loss of sections from slides.

For observation of cell files along the root axis,whole roots were cleared and stained in lactic acid with fluorescence stains (berberine or fluorol yellow) and post-stained with safranin O.This procedure allows observation of epidermal and exodermal cells in thick roots of various species, and in thin roots of arabidopsis it works well for staining and observation of endodermal cells. In thick roots such as those of maize or sorghum, peeling peripheral root tissues exposes the endodermal cells and allows their direct observation. The contrast of cell walls is increased after treatment of peeled samples with berberine in lactic acid.

圖1.「D-H」甜瓜不定根的切片經透明處理和含熒光黃088的乳酸染色液染色，在白光「D」、紫外光「E」和兩種光下的重疊圖片「F」。

文獻2: Cohen H et al. A Multilevel Study of Melon Fruit Reticulation Provides Insight into Skin Ligno-Suberization Hallmarks. Plant Physiol. 2019 Apr;179(4):1486-1501.

A）Histochemical observations of suberin and lignin in skin cross sections were achieved by staining with a freshly prepared solution of Fluorol Yellow 088 (0.01% [w/v] in lactic acid) for 30 min at 70°C and phloroglucinol-hydrochloric acid (2% [w/v] in 50% hydrochloric acid) for 30 min at room temperature, respectively. Stained sections were then observed with an Olympus CLSM500 microscope using bright-field and GFP filters. Autofluorescence of cuticle layers in smooth skin sections was observed with a Cy5 filter.

圖2.光滑和網狀果皮橫截面切片的木質素（lignin）（間苯三酚-鹽酸染色）和木栓質（suberin）（熒光黃088）染色。

文獻3: Gunawardena AH et al. Cell wall degradation and modification during programmed cell death in lace plant, Aponogeton madagascariensis (Aponogetonaceae). Am J Bot. 2007 Jul;94(7):1116-28.

A）For fluorol yellow 088, a final concentration of 0.01% (w/v) was made by dissolving 0.01 g of fluorol yellow 088 in 50 mL of PEG 400 solution and heating at 90°C for 1 h (Brundrett et al., 1991). An equal volume of 90% (v/v) glycerol solution was added to the PEG staining solution. Leaves were stained for 1 h at room temperature, rinsed several times with distilled water, and observed with UV light for a bright yellow fluorescence. Unstained cell walls were also examined for autofluorescence as a test for the phenolic constituents of suberin.

圖3：成熟花邊植物葉的細胞壁組織化學檢測。熒光黃088染脂肪族物質「A-D」。透化葉子（A，B）；新鮮葉子（C，D）。微分干涉相襯顯微成像圖片觀察到棕色色素，而熒光成像圖片觀察到陽性染色（黃綠色熒光）。

注意事項

1）本品僅用作科研用途，絕不可用于臨床診斷或治療藥物，絕不可用做食品或藥品。

2）為了您的安全和健康，請穿實驗服并戴一次性手套操作。

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上海懋康生物科技有限公司是一家涉足于生命科學和生物技術領域研究的試劑、儀器和實驗室消耗品與實驗服務工作，主要從事細胞生物學、植物學、分子生物學、免疫學、生物化學、蛋白組學。生物制藥與診斷試劑研發生產等領域。 本公司秉承“以人為本，以誠為信、合同守信”的經營理念。堅持"品質保障"的原則為廣大客戶提供優質產品。