產品名稱

產品編號

CAS NO.

規格             

價格（元）   

Calyculin A    花萼海綿體誘癌素A    

MZ2504-25UG        

101932-71-2   

25 μg

4188

Calyculin A   花萼海綿體誘癌素A

MZ2504-100UG

101932-71-2

100 μg

8498

文獻1，Hudák R et al. The Phosphatase Inhibitor Calyculin-A Impairs Clot Retraction, Platelet Activation, and Thrombin Generation. Biomed Res Int. 2017:9795271.PMID: 28680886

體外研究：

細胞類型（Cell type）：Platelet-rich plasma (PRP) sample

藥物配制（Preparation）：Calyculin-A (CLA) was dissolved in DMSO

實驗方法（Assay）：PRP (110μL) was preincubated with either HEPES buffer containing 0.5% DMSO as control or the protein phosphatase inhibitor CLA (50nM), for 30 minutes at 37°C in a water bath. After preincubation, platelets were activated either by TRAP (20μM) or by thiomersal (200mM) for 15 minutes at 37°C in a water bath. Then PRP (5μL) was stained with 5μL monoclonal CD41-PE antibody and 5μL Annexin-V-FITC and Annexin-V binding buffer (1x concentrate) was added to examine the PS-expression of the platelets.

文獻2，Blank T et al. The phosphoprotein DARPP-32 mediates cAMP-dependent potentiation of striatal N-methyl-D-aspartate responses. Proc Natl Acad Sci U S A. 1997 Dec 23;94(26):14859-64. PMID: 9405704

體外研究：

細胞類型（Cell type）：Xenopus Oocytes

藥物配制（Preparation）：Calyculin A was prepared in 100% ethanol as a 1 mM stock solution. Immediately after dilution in distilled water to the appropriate concentration, calyculin A was microinjected into the oocytes according to the RNA injection protocol.

實驗方法（Assay）：The NMDA-induced currents of striatal mRNA-injected oocytes were measured before and 8 min after 1-min application of 50 μM forskolin or before and 12 min after 1-min application of 10 nM PMA. The same protocol was followed after injecting oocytes with calyculin A to a final intracellular concentration of 500 nM 60–90 min before voltage-clamping. The intracellular concentration of calyculin A was calculated by assuming standard oocytes with a volume of 0.5 μl.

文獻3，Fabian L et al. Calyculin A, an enhancer of myosin, speeds up anaphase chromosome movement. Cell Chromosome. 2007 Mar 24;6:1. PMID: 17381845

體外研究：

細胞類型（Cell type）：Living crane-fly spermatocytes

藥物配制（Preparation）：Calyculin A was dissolved in DMSO as a 1mM or 50 μM stock solution.

實驗方法（Assay）：Living crane-fly spermatocytes were perfused with insect Ringer's solution or with Calyculin A in insect Ringer's solution, at final concentrations of 0.5 μM, 100 nM, 50 nM, 20 nM, 10 nM or 5 nM, prepared from a 1 mM or a 50 μM Calyculin A stock in DMSO. Results showed that concentrations of 5 nM and 10 nM had no effect on anaphase chromosome movement, 20 nM had inconsistent effects, and 50 nM had consistent effects. Thus we used 50 nM for most of the experiments.

貨號

名稱

規格            

MZ2502-25UG            

Calyculin A 花萼海綿體誘癌素A

25 μg

MZ2401-100UL

PMA (TPA, 5mg/ml) 佛波肉荳蔻醋酸（5mg/ml）  

100μl

MZ2403-1MG

4α-PMA (4α-TPA) 4α-佛波肉荳蔻醋酸

1mg