BMPO (Spin trapping reagent)自旋捕獲劑

產品標簽

BMPO；DEPMPO；EMPO；Spin trapping reagent自旋捕獲劑；電子順磁共振波譜（EPR）；CAS: 387334-31-8；

產品信息

產品描述

BMPO（5-tert-butoxycarbonyl 5-methyl-1-pyrroline N-oxide）是一種新型高效且高穩定型的硝酮自旋捕獲劑（Nitrone Spin Trapping Reagent），由美國威斯康星醫學院Kalyanaraman教授的實驗室團隊開發，非常適合用于特異性檢測和鑒定體內外形成的硫自由基、羥基自由基（?OH）和超氧陰離子自由基（?O2-），通過形成用電子順磁共振波譜（EPR）可區分的加合物來測定[1-2]。

BMPO具有以下特點：

①特異性高，半衰期長：其它的硝酮自旋捕獲劑比如：DMPO，難以輕易區分超氧陰離子和羥基自由基，因產生的DMPO-超氧加合物（半衰期t?=45s）瞬間衰減產生DMPO-羥基加合物。與最近開發的自旋捕獲劑：DEPMPO和EMPO類似，BMPO-超氧加合物不會衰減生成羥基加合物，但是，BMPO-超氧加合物的半衰期最長（t?=23min）。

②產品穩定性高：DEPMPO和EMPO是液體自旋捕獲劑，通常被硝基氧雜質污染，且效期有限。BMPO是固體的環形硝酮，通過結晶以高度純化的狀態提供，能夠保存更長的周期，且不用擔心降解。

③更高的信噪比：BMPO衍生的加合物在各自的EPR光譜中具有更高的信噪比，使其更適合用于檢測細胞懸浮液中的亞硫酸、羥基和甲基自由基。

④高水溶性：水溶性好，更利于水相體系自由基的研究，尤其是生物體系的自由基研究。

產品特性

1） CAS NO：387334-31-8

2） 化學名：3,4-dihydro-2-methyl-1,1-dimethylethyl ester-2H-pyrrole-2-carboxylic acid-1-oxide

3） 同義名：5-tert-butoxycarbonyl 5-methyl-1-pyrroline N-oxide; BocMPO;

4） 分子式：C10H17NO3

5） 分子量：199.2

6） 純度：≥98%

7） 外觀：結晶或結晶性粉末

8） 溶解性：溶于水、PBS（pH 7.2, 10mg/ml）、DMSO（25mg/ml）、無水乙醇（25mg/ml）

8）化學結構式：

保存與運輸方法

保存：-20oC干燥保存，2年有效。 

運輸：冰袋運輸。

注意事項

為了您的安全和健康，請穿實驗服并戴一次性手套操作。

參考文獻

1. Zhao, H., Joseph, J., Zhang, H., et al. Synthesis and biochemical applications of a solid cyclic nitrone spin trap: A relatively superior trap for detecting superoxide anions and glutathiyl radicals. Free Radic. Biol. Med. 31(5), 599-606 (2001).

2. Khan, N., Wilmont, C.M., Rosen, G.M., et al. Spin traps: In vitro toxicity and stability of radical adducts. Free Radical Biology & Medicine 34(11), 1473-1481 (2003).

應用示例（僅作參考）

1.文獻來源：Mitchell DG, Rosen GM, Tseitlin M, Symmes B, Eaton SS, Eaton GR. Use of rapid-scan EPR to improve detection sensitivity for spin-trapped radicals. Biophys J. 2013 Jul 16;105(2):338-42. doi: 10.1016/j.bpj.2013.06.005. PMID: 23870255; PMCID: PMC3714875. Editedby MKBIO

①黃嘌呤-黃嘌呤氧化酶體系內超氧陰離子（O2??）生成的測定：Typically, xanthine oxidase (0.04 U/mL) was added to pH ～7.4 sodium phosphate buffer (50 mM) containing DTPA (1 mM) and hypoxanthine (0.5–400 μM, final concentration) to achieve rates of O2?? formation that ranged from 0.1 to 6.0 μM/min. We estimated the superoxide formation rate by monitoring the SOD-inhibitable reduction of ferricytochrome c (80 μM) at room temperature. Spin trapping was performed by addition of 100 mM BMPO in pH ～7.4 phosphate-buffered saline (PBS; 50 mM) containing 1 mM DTPA to the solution of hypoxanthine and xanthine oxidase to achieve a final BMPO concentration of 50 mM in the reaction mixture. EPR spectra were recorded 10 min after mixing reagents. The half-life of BMPO-OOH at ambient temperature is reported to be ～23 min. Solutions for control experiments contained SOD (30 U/mL).

Figure 2 Comparison of CW and rapid-scan spectra of BMPO-OOH in solution with a O2?? production rate of 0.1μM/min, recorded 10 min after mixing reagents. The O2?? was produced by a hypoxanthine/xanthine oxidase mixture. The concentration of BMPO-OOH is ～0.3μM. (A) CW spectrum obtained with 55 G sweep width, 0.75 G modulation amplitude, single 30 s scan, 15 ms conversion time, 10 ms time constant, and 20 mW (B1 = 170 mG) microwave power. (B) Deconvolved rapid-scan spectrum obtained with 55 G scan width, 51 kHz scan frequency, and 53 mW (B1 = 250 mG) microwave power. Segments consisting of 12 sinusoidal cycles were averaged 100 k times, with a total data acquisition time of ～30 s.

2.文獻來源：Wang, Z., Zhang, Y., Ju, E.et al. Biomimetic nanoflowers by self-assembly of nanozymes to induce intracellular oxidative damage against hypoxic tumors.Nat Commun 9,3334 (2018). https://doi.org/10.1038/s41467-018-05798-x 

①缺氧下的ESR測定（O2??，?OH）：For O2?? detection, the pre-deoxidized PBS (pH 5.0, 25?mM) contained 25?mM BMPO, 20?μg?mL?1 MnO2@PtCo nanoflowers, 100?μM H2O2, 50% DMSO was prepared. After incubation of 5?min, ESR spectra were recorded. For ?OH detection, the pre-deoxidized PBS (pH 5.0, 25?mM) contained 25?mM BMPO, 20?μg?mL?1 MnO2@PtCo nanoflowers, 100?μM H2O2, 0.25?U?mL?1 SOD was prepared. After incubation of 5?min, ESR spectra were recorded. The following instrument settings were used for collecting ESR spectra: 1?G field modulation, 100?G scan range, and 20?mW microwave power.

Fig f ESR spectra of BMPO/?OOH adducts from different groups in the hypoxic H2O2 (100?μM) condition upon addition of DMSO.g ESR spectra of BMPO/?OH adducts were collected from different samples in the hypoxic H2O2 (100?μM) condition upon addition of SOD. Data were presented as mean?±?s.d. (n=?5).

相關產品

 — —Written/Edited by V. Shallan【版權歸MKBio懋康所有】

上海懋康生物科技有限公司是一家涉足于生命科學和生物技術領域研究的試劑、儀器和實驗室消耗品與實驗服務工作，主要從事細胞生物學、植物學、分子生物學、免疫學、生物化學、蛋白組學。生物制藥與診斷試劑研發生產等領域。 本公司秉承“以人為本，以誠為信、合同守信”的經營理念。堅持"品質保障"的原則為廣大客戶提供優質產品。